RNAseq of mCherry positive cells at day 40 after transplantation - SRA

SRX25688898: RNAseq of mCherry positive cells at day 40 after transplantation
1 ILLUMINA (NextSeq 2000) run: 24.4M spots, 1.7G bases, 557.8Mb downloads

Design: Cells of whole animal recipient were dissociated and mCherry positive cells were FACS sorted. RNA was extracted using TRIzol. Library was constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs).

Submitted by: The Hebrew University of Jerusalem

Study: Candidate Stem Cell Isolation and Transplantation in Hexacorallia

PRJNA1147772 • SRP526147 • All experiments • All runs

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We aimed to study the capacity of candidate stem cells in Nematostella vectensis to differentiate upon transplantation into a recipient animal. TBP::mCherry transgenic animals were used as donors. Cells were isolated from the mesentery of a donor and RNA was extracted. 40 days upon transplantation recipient animals were dissociated and mCherry positive cells were isolated by FACS. RNA was then extracted and sequenced. We then compared the transcriptomes before and after transplantation.

Sample: mCherry positive cells of the recipient at 40 days after transplantation.

SAMN43168294 • SRS22331041 • All experiments • All runs

Organism: Nematostella vectensis

Library:

Name: mp3

Instrument: NextSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: SINGLE

Runs: 1 run, 24.4M spots, 1.7G bases, 557.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR30226458	24,416,801	1.7G	557.8Mb	2024-09-01

ID:: 34711665

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